test2(2)

test2(2) - BCH 4053 June 30, 2000 HOUR TEST 2 NAME_ Page...

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BCH 4053 June 30, 2000 HOUR TEST 2 NAME_____________________ (10) 1. Given the following data on three different proteins: Protein: hemoglobin chymotrypsinogen urease Molecular Weight (M) 64,500 23,250 482,000 Diffusion Coefficient (D) 6.9 9.5 3.5 Isoelectric pH (pI) 6.8 9.5 5.0 Page Points 1 ____ 2 ____ 3 ____ 4 ____ Total ______ Indicate in the blanks which of the three proteins will: __________________(a) Elute first from a gel filtration column. __________________(b) Elute first from a diethylaminoethyl cellulose ion exchange column. __________________(c) Have the smallest frictional coefficient (f). __________________(d) Migrate fastest upon electrophoresis in sodium dodecyl sulfate (SDS). __________________(e) Migrate fastest to the anode in a native electrophoresis experiment at pH 6.0. (6) 2. For the following common secondary structures of a protein, identify the Φ and Ψ angles associated with that structure from the list in the middle. Place the letter corresponding to the correct Φ and Ψ angles in the blank to the left of the secondary structure. (A Ramachandran map is shown at the right for reference to angle values). _____ alpha helix _____ beta sheet _____ collagen helix a. Φ = -51 o Ψ = 153 o b. Φ = 90 o Ψ = -90 o c. Φ = -57 o Ψ = -47 o d. Φ = 180 o Ψ = 0 o e. Φ = -139 o Ψ = 135 o (6) 3. Fill in the following blanks with á helix or disordered to represent the primary conformation
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This note was uploaded on 05/22/2011 for the course BCH 4053 taught by Professor Logan during the Fall '06 term at FSU.

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test2(2) - BCH 4053 June 30, 2000 HOUR TEST 2 NAME_ Page...

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