test2 - BCH 4053 June 29, 2001 HOUR TEST 2 NAME_ Page (4)...

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BCH 4053 June 29, 2001 HOUR TEST 2 NAME_____________________ (4) 1. In the Edman degradation of a protein, phenyl isothiocyanate is reacted with a peptide in ___________(acid or alkaline?) conditions, and the _________(amino or carboxyl?) terminal aminoacid is cleaved in ___________(acid or alkaline?) conditions and isolated and analyzed as a(n)____________________(name) derivative. Page Points 1 ____ 2 ____ 3 ____ 4 ____ 5 ____ Total ______ (9) 2. Explain the role of each of the following reagents in sequencing a protein: (a) cyanogen bromide (b) fluorodinitrobenzene (Sanger reagent) (c) performic acid (6) 3. (a) On the figure at the right, label the F (phi) and Y (psi) rotation angles. (b) Draw a Ramachandran map below, label the axes properly, and indicate on the map the conformational location of (a) an alpha helix, (b) a beta sheet, and (c) collagen. Shade the quadrant of the map in which most F , Y angle combinations are found in proteins.
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BCH 4053 --Hour Test 2 Page 2 Name________________________ (13) 4. Given the following data on five different proteins: Protein M.W.(x10 -3 ) D pI fibrinogen 330 2.0 5.8 hemoglobin 64.5 6.9 7.0 lysozyme 14.4 11.2 11.0 ovalbumin 45.0 7.8 4.6 urease 483 3.5 5.0 Assume all proteins have partial specific volumes of about 0.73 ml/g.
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test2 - BCH 4053 June 29, 2001 HOUR TEST 2 NAME_ Page (4)...

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