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DNA1 PPT s11 - Lab15DNATechnologyI PlasmidDNAIsolation Rev...

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Click to edit Master subtitle style Lab 15 - DNA Technology I:  Plasmid DNA Isolation  Rev. 5/22/11
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The Case  (LM p. 31-32) Defendants  (Parents and their young daughter) Acquired an infection believed to be nosocomial The infection led to complications and the need for an additional surgery The additional surgery was not covered by the parents’ insurance The parents were suing the hospital for the payment The parents state that many patients on the ward had a similar infection Plaintiff  (Hospital) The case is an isolated incident Mask and gowning was a precautionary measure The parents should pay for the surgery in full Court Ruling Test the volunteer patients A, B, C, and D from the defendant’s ward If at least one of the volunteers has a similar infection, testing of all patients will be mandated
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Introduction DNAI Purpose : Isolate plasmids from bacteria Received 2 bacterial cultures containing plasmids. These bacteria were cultured originally from patient A, B, C, and D. Isolated plasmid DNA from two of these cultures. DNAII Purpose : Identify (map) the plasmid DNA molecules Cut plasmid DNA samples with EcoRI and PvuII restriction endonucleases.
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