DNA1 PPT s11 - Lab15DNATechnologyI: PlasmidDNAIsolation

Info iconThis preview shows pages 1–4. Sign up to view the full content.

View Full Document Right Arrow Icon
Click to edit Master subtitle style Lab 15 - DNA Technology I:  Plasmid DNA Isolation  Rev. 5/22/11
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
The Case  (LM p. 31-32) Defendants  (Parents and their young daughter) Acquired an infection believed to be nosocomial The infection led to complications and the need for an additional surgery The additional surgery was not covered by the parents’ insurance The parents were suing the hospital for the payment The parents state that many patients on the ward had a similar infection Plaintiff  (Hospital) The case is an isolated incident Mask and gowning was a precautionary measure The parents should pay for the surgery in full Court Ruling Test the volunteer patients A, B, C, and D from the defendant’s ward If at least one of the volunteers has a similar infection, testing of all patients will be mandated
Background image of page 2
Introduction DNAI Purpose : Isolate plasmids from bacteria Received 2 bacterial cultures containing plasmids. These bacteria were cultured originally from patient A, B, C, and D. Isolated plasmid DNA from two of these cultures. DNAII Purpose : Identify (map) the plasmid DNA molecules Cut plasmid DNA samples with EcoRI and PvuII restriction endonucleases. Run the digested DNA on an agarose gel.
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 4
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 10

DNA1 PPT s11 - Lab15DNATechnologyI: PlasmidDNAIsolation

This preview shows document pages 1 - 4. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online