DNA2 PPT s11 student - Lab 16 DNA Technology II Restriction...

Info icon This preview shows pages 1–4. Sign up to view the full content.

View Full Document Right Arrow Icon
Lab 16 - DNA Technology II: Restriction Endonucleases Rev. 2/21/2011
Image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
The Case (LM p. 31-32) Defendants (Parents and their young daughter) Acquired an infection believed to be nosocomial The infection led to complications and the need for an additional surgery The additional surgery was not covered by the parents’ insurance The parents were suing the hospital for the payment The parents state that many patients on the ward had a similar infection Plaintiff (Hospital) The case is an isolated incident Mask and gowning was a precautionary measure The parents should pay for the surgery in full Court Ruling Test the volunteer patients A, B, C, and D from the defendant’s ward If at least one of the volunteers has a similar infection, testing of all patients will be mandated
Image of page 2
Introduction DNAI Purpose : Isolate plasmids from bacteria Received 2 bacterial cultures containing plasmids. These bacteria were cultured originally from patient A, B, C, and D. Isolated plasmid DNA from two of these cultures. DNAII Purpose : Identify (map) the plasmid DNA molecules Cut plasmid DNA samples with EcoRI and PvuII restriction endonucleases. Run the digested DNA on an agarose gel. Determine the size of the plasmid fragments in base pairs. Compare the restriction patterns of the plasmids with that of the restriction patterns of the plasmid from the defendant’s infection.
Image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 4
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern