L21 Protocol Overview student

L21 Protocol Overview student - Lab 21 Protocol Overview An...

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1 Lab 21 Protocol Overview An overview of all of the exercises for Lab 21 is given here based on LabTutor. Use it to highlight the critical activities for the lab. 1. You will start Lab Activity 2: Frog Skeletal Muscle first. It is essential that throughout this Laboratory you observe what is actually happening to the muscle. You are expected to describe your observations and these descriptions will be included in your Lab Tutor report. 2. * START * with the EQUIPMENT and Force TRANSDUCER SETUP, not the Frog dissection. A. Activity 2: Frog Skeletal Muscle Equipment Setup o Connect the Bridge Pod to Input 1 of the PowerLab. o Connect the positive and negative BNC connectors of the stimulating electrode leads to the analog Outputs on the PowerLab. Force Transducer setup o Raw output from the force transducer is millivolts. It needs to be calibrated to give the more meaningful units of Newtons (N). o Force transducers also often have some residual offset voltage that needs to be corrected for. Frog Skeletal Muscle Dissection o You will cut the skin midway the abdomen of the frog, and then remove it to expose the leg muscles. o You will isolate the gastrocnemius muscle with distal tendon attached (for securing to the Z-pin) and femur attached (for securing in the muscle holder).
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This note was uploaded on 05/22/2011 for the course BIO 205 taught by Professor O'neal during the Spring '08 term at SUNY Stony Brook.

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L21 Protocol Overview student - Lab 21 Protocol Overview An...

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