TFNPCR assignment s11

TFNPCR assignment s11 - Lab 24/25 DNA III/IV In-class Group...

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Lab 24/25 DNA III/IV In-class Group Assignment Section # ____ Group # ____ Print Group Members’ Names: Transformation Results (14) 1. Check the box after adding your group transformation data to the class Excel spreadsheet ± 2. Use the Letters of the plates to answer the following questions. Designate the RM DNA # _______. A B C D E E. coli + TE on LB E. coli + TE on LB/amp E. coli + RM on LB E. coli + RM on LB/amp E. coli + RM on LB/amp/ara Plate Letters List the plate(s) that show(s) the original E. coli are ampicillin sensitive: List the plate(s) that display(s) the total number of E. coli cells: List the plate(s) on which only transformants will grow: List the plate(s) that contain(s) recombinants: List the plate(s) on which only recombinants will grow: 3. Percentage of E. coli with pGLO on plate E : / * 100 = % 4. Percentage of E. coli with pBAD18 on plate E : / * 100 = % “Thought Experiment “ Data Interpretation (data posted in Bb section) Use results of “Thought Experiment” for Q 5-10. 5.
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This note was uploaded on 05/22/2011 for the course BIO 205 taught by Professor O'neal during the Spring '08 term at SUNY Stony Brook.

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TFNPCR assignment s11 - Lab 24/25 DNA III/IV In-class Group...

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