8-DNA-Repl-Repair - DNA Replication Chapter 6 Dr Beth...

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1 DNA Replication Chapter 6 Dr. Beth Dawson BIO 3, Fall 2010 San José State University
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2 How does DNA replicate? Figure 6-6 Essential Cell Biology (© Garland Science 2010)
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3 DNA Replication Meselson and Stahl – Grew E. coli with 15 N – Put into 14 N medium for 1 replication
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4 DNA Replication Meselson and Stahl – Result: DNA had intermediate density – Consistent with semiconservative replication
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5 DNA Replication Figure 6-3 Essential Cell Biology (© Garland Science 2010)
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6 Key Enzymes and Proteins 1. DNA Polymerase 2. Ligase 3. Primase 4. Telomerase 5. Helicase 6. SSBPs 7. Topoisomerase
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7 Key Enzymes and Proteins DNA Polymerase Catalyzes polymerization Adds to 3’ end Can only add to 3’ OH group Can “back up” for repair Vary between eukaryotes and prokaryotes
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8 Key Enzymes and Proteins • Ligase Forms covalent bonds to join adjacent strands Okazaki fragments • Primase Synthesizes short RNA primers RNA polymerase Binds to helicase
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9 Key Enzymes and Proteins • Telomerase Adds DNA to 3’ end Uses its own internal RNA sequence 5’--TTAGGG in all vertebrates 3’ ends of chromosomes have lots of those repeats Discoverers awarded Nobel Prize in 2009
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10 Key Enzymes and Proteins • Helicase – Separates double-stranded DNA – Replication, transcription, recombination and DNA repair and other – A class of enzymes – Uses energy from hydrolysis of ATP
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11 Key Enzymes and Proteins • SSBP – Single-stranded binding proteins – Associates with single-stranded DNA – Prevents re-annealing – Stabilizes single-stranded DNA
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