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Bio3-DNA-Fill-in-the-blanks - Bio 3 Spring 2009 San Jos...

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Bio 3, Spring 2009 San José State University Dr. Dawson “Fill in the blanks” Study Guide for DNA Replication and Transcription/Translation DNA replication occurs in the ________________when the cell is dividing. In order for the DNA replication machinery to do its job, the DNA template must be single stranded. Therefore, the double- stranded DNA molecule needs to be unwound by the enzyme ________________. But unwinding the DNA strains the molecule ahead of the replication fork. Another enzyme called ________________ associates with the DNA ahead of the replication fork and relieves the DNA supercoiling. The unwound portions of DNA are stabilized by proteins called ________________________________. Now that we have single-stranded DNA, we need one more thing before replication can begin. The enzyme that creates new ________________ bonds between adjacent nucleotides can only add to the ______end of an existing strand. Primase solves this problem by making a ________________ of (DNA / RNA). This short string is about 5-10 base pairs long. The enzyme (DNA / RNA) polymerase then adds new nucleotides in a condensation reaction that forms a ________________ bond between the two nucleotides. This process is (exergonic / endergonic) and is coupled to the dissociation of a
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