Capillary Electrophoresis

Capillary Electrophoresis - Capillary Electrophoresis By:...

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Click to edit Master subtitle style 5/24/11 Capillary Electrophoresis By: Waepril Chua 11
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5/24/11 Outline Introduction/Background of Capillary Electrophoresis (CE) Instrumentation Setup Electroosmotic Flow (EOF) Data Collection 22
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Capillary Electrophoresis Capillary electrophoresis - technique used to separate ions or solutes using capillary tubes, which are about 25 to 100 µm in internal diameter. - separation occurs due to differences in the migration velocity of different solutes. v=µeE where E=electric field, µe=electrophoretic mobility - velocity is proportional to the electric field - electrophoretic mobility- measure of how fast an analyte will travel in a given buffer/medium µe = ___q_ _ where q=charge of the ion, r=ionic radius, 6 πηr η= solution viscosity Skoog, D.A.; Holler, F.J.; Crouch, S.R. Principles of Instrumental Analysis , 6th ed; Thomson Brooks/Cole Publishing: Belmont, CA, 2007; Chapter 30. 33
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Capillary Electrophoresis - Capillary Electrophoresis By:...

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