Lec21 - Lecture 21 Eukaryotic Gene Regulation 3...

Info icon This preview shows pages 1–7. Sign up to view the full content.

View Full Document Right Arrow Icon
Lecture 21 Eukaryotic Gene Regulation 3 Transcription Repression & Combinatorial Gene Control (Lodish 6th ed. Chapter 7) (L.5th edition Chapter 11)
Image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Transcription repressors are modular Flexible hinge DNA (recognition of specific DNA sequences) Repression domain DNA-binding domain core promoter GTFs & Pol II CACGTG Repressor-specific DNA recognition sequence “repression” GTFs = general transcription factors Repressor
Image of page 2
A B C TBP x Transcription Repression Mechanisms “quenching” TBP TAFs TFIID TAFs Prevents activators from contacting general transcription factors Prevents assembly of other GTFs and Pol II into a complete pre- initiation complex (PIC).
Image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Recruitment of corepressors that modify histones HDAC E D Heterodimerization with dominant- negative inhibitory factor (e.g. Id ) repressor corepressor Deacetylated nucleosomes tightly packed over TATA box e.g.:HDAC = h istone d eac etylase F Recruitment of corepressors that contact the general transcription machinery TATA Inhibitor (e.g.: Id, an inhibitor of MyoD) x Activator dimer (e.g. MyoD) Binding site for activator TATA Binding site for repressor TBP CoR Corepressor inhibits pre-initiation complex (PIC) assembly (e.g.: NC2, negative cofactor 2) Binding site for repressor x GTFs/Pol II TBP x Excess inhibitor
Image of page 4
Sin3-HDAC complex HDAC HDAC = Histone deacetylase Lodish 6th. Ed. Fig. 7-38
Image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
SAGA complex HAT HAT = Histone acetyl-transferase Lodish 6th. Ed. Fig. 7-38
Image of page 6
Image of page 7
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern