Biol522LabEx6

Biol522LabEx6 - Name_ Pre-Laboratory Exercise Laboratory 6...

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Name______________________ Pre-Laboratory Exercise Laboratory 6 Suppose that substance IGLOO is required for the formation of the bacterial cell wall. It is made by the following pathway: A B C D Ig + L Igl + u Iglu Iglou Igloo A bacterium which is unable to make enzyme A will be unable to grow. Will it grow if I feed it Igl? _______ Will a bacterium which can't make enzyme C grow if I feed it Igl? ________ The A bacterium accumulates Ig. Will it be able to cross feed C? _______ The C bacterium accumulates Iglu. Will it be able to crossfeed A? _____
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Biology 422L Fall, 2006 Laboratory 6 Biochemical Pathways Objectives : 1) To become familiar with the growth characteristics and interactions of mutant bacteria blocked in a particular biochemical pathway. 2) To experience how bacterial mutants can be used to help to elucidate a particular pathway. 3) To become familiar with the concept of a biochemical pathway and to understand the implications of this concept. References : Madigan et al, p.131, any biochemistry textbook, and the biocyc web site http://biocyc.org/server.html Auxotrophic mutants have been used to help elucidate the pathway for the biosynthesis of many compounds. You will be provided with a set of four auxotrophic mutants which require
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Biol522LabEx6 - Name_ Pre-Laboratory Exercise Laboratory 6...

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