NAME ___________________________BIOLOGY 205/SECTION 7DEVELOPMENT- LiljegrenProblem set 121.) You’re a scientist in a lab studying Waardenburg’s syndrome I and II.a)What gene(s) could you knockout to generate mouse models of these syndromes?____Pax3, Mitf__________________________b)ES cells, which are crucial for this procedure, are derived from what cell type inblastocyst embryos? ____inner cell mass___________________c)What rare event in ES cells will replace one wildtype copy of your selected genewith your “knockout” gene? ____homologous recombination_______________d)How would you identify the ES cells in which this rare event had occurred?Insert neomycin marker into your gene to provide a way to select for whetherknockout allele is present. After introducing your knockout plasmid into ES cells,grow them with neomycin in the media to select for cells in which rare homologousrecombination had replaced the endogenous gene with your knockout version.Cells that have knockout allele would be able to survive, cells without knockoutallele incorporated would die.
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