DBLecture10posted

DBLecture10posted - Model organisms: mice vertebrates! mice...

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Model organisms: mice vertebrates! mice are ~ 3 inches long, can keep many mice in a room. generation time is ~ 3 months, so genetics can be done history - scientists have worked with mice for 100 years genetic tools - can introduce extra genes or remove a specific gene, then study the effect on development Disadvantages: development inside the mother, hard to see. Expensive! Large Genome = 3 Gb
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The mouse provides a superb model for human development and disease because we share virtually ALL of our genes and use them in similar ways Figure 1.22 Kit gene
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Genetic analysis: creating transgenic mice Problem: Find a cell line that can grow in tissue culture but also retains the potential to become part of a real embryo. Solution: Embryonic stem cells
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Embryonic stem cells: blastocyst-stage cells (from inner cell mass) that have been coaxed into growing in culture blastocyst inner cell mass
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Blastocyst stage cells can be easily incorporated into a different blastocyst stage embryo, allowing production of chimeric mice Fig. 8.26 mouse with 4 parents!! mom and dad have white fur mom and dad have black fur
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A mouse with 3 of its parents (6 total!) Fig. 8.26
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Adding a gene: Producing Transgenic Mice
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Production of Transgenic Mice Embryonic stem cells (ES cells) are then incorporated into blastocysts, with the hope that they “go germline”.
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DBLecture10posted - Model organisms: mice vertebrates! mice...

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