ExamIII2006key

ExamIII2006key - Biology 52 Exam III, April 5, 2006 1. i)...

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Biology 52 Exam III, April 5, 2006 1. i) arrest (haploid) ii) develop iii) arrest (DNA methylation, histone modification, both of which affect gene expression) iv) develop c) Electric pulse fuses cell membranes together and activates oocyte (cell divisions begin) d) G0 cells have the correct DNA content (2N, 2C) and there is a delay into cell cycle entry 2. PLC-zeta cleaves PIP2 (a phospholipid) into IP 3 and DAG. IP 3 promotes Ca ++ release from ER lumen which initiates the cortical reaction; the egg is then activated (cell divisions begin) 3. a) b) i) secreted signal; ii) BMP-4 antagonist; iii) cell-cell attachment; iv) transcription factor c) UV irradiation prevents cortical rotation; tadpole will not have dorsal axis e) Xnr inject early into dorsal vegetal cells; noggin & goosecoid inject into dorsal mesoderm 4. a) b) Purify organizer cells by FACS (fluorescence activated cell sorting) or by dissection, isolate RNA, make labeled cDNA probe, hybridize to array, compare to control (non-organizer) tissue
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This note was uploaded on 05/26/2011 for the course BIO 205 taught by Professor Reed during the Spring '11 term at UNC.

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ExamIII2006key - Biology 52 Exam III, April 5, 2006 1. i)...

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