Lecture 3 - Cell potential and cloning

Lecture 3 Cell - Cell potential animal cloning Can any cell develop into a complete organism How do you clone a sheep Regenerating plants from a

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Cell potential, animal cloning Can any cell develop into a complete organism? How do you clone a sheep?
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Regenerating plants from a single cell
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Testing the developmental potential of nuclei from differentiated cells (Needle prick can activate some eggs) (or suck out meiotic spindle with pipette) Does tadpole develop?
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Copyright ©2003 by the National Academy of Sciences Gurdon, J. B. and Byrne, J. A. (2003) Proc. Natl. Acad. Sci. USA 100, 8048-8052 Fig. 2. (A) In Amphibia, nuclear transfer success declines rapidly as cells differentiate
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Copyright ©2003 by the National Academy of Sciences Gurdon, J. B. and Byrne, J. A. (2003) Proc. Natl. Acad. Sci. USA 100, 8048-8052 Fig. 2. (A) In Amphibia, nuclear transfer success declines rapidly as cells differentiate
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Donor nucleus Stages reached Zygote blastula, tadpole, adult Blastula blastula, tadpole, adult Tadpole intestine blastula, tadpole, adult Cultured skin cell blastula (differentiated) Success of nuclear transplant experiments
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Donor nucleus Stages reached Zygote blastula, tadpole, adult Blastula blastula, tadpole, adult Tadpole intestine blastula, tadpole, adult blastula Blastula from above blastula, tadpole Could some genes be missing from differentiated skin cells?
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Serial nuclear transfer improves the efficiency of cloning
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Conclusions of nuclear transplantation experiments in amphibians: 1) Nuclei of differentiated cells have the potential to program development of many or all cells. (Therefore, the nuclei must contain all the necessary genes.)
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Conclusions of nuclear transplantation experiments in amphibians:
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This note was uploaded on 05/26/2011 for the course BIO 205 taught by Professor Reed during the Spring '11 term at UNC.

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Lecture 3 Cell - Cell potential animal cloning Can any cell develop into a complete organism How do you clone a sheep Regenerating plants from a

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