Lecture 5 - Axis determination in frog embryos

Lecture 5 - Axis determination in frog embryos - Axis...

Info iconThis preview shows pages 1–9. Sign up to view the full content.

View Full Document Right Arrow Icon
Axis determination in frog embryos How are embryonic axes set up? What signaling events regulate embryo development?
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Time-lapse videos of Xenopus gastrulation: By David Shook : http://faculty.virginia.edu/shook/ShowMovies/index.htm By Mike Danilchik: http://worms.zoology.wisc.edu/frogs/gastxen/gastxen_sagview.html
Background image of page 2
Overview of frog gastrulation Future Dorsal side (back) Sperm entry in animal hemisphere
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Vg1 mRNA ( in situ hybridization)
Background image of page 4
A 3’ UTR sequence element localizes Vg1 mRNA (From Mowry and Melton (1992) Science 255: 991-993) i) Inject RNA molecules into oocyte ii) Isolate animal and vegetal RNAs iii) Northern blot using Vg1 probe
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
A 3’ UTR sequence element localizes Vg1 mRNA (depends on microtubules, mRNA-binding proteins) (From Mowry and Melton (1992) Science 255: 991-993)
Background image of page 6
Sperm entry provides the asymmetric cue to set up the dorsal-ventral axis of Xenopus embryos Cortical rotation requires the microtubules – may be nucleated by the centriole delivered by sperm.
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
UV irradiation on vegetal side prevents cortical rotation.
Background image of page 8
Image of page 9
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 30

Lecture 5 - Axis determination in frog embryos - Axis...

This preview shows document pages 1 - 9. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online