Drosophilia Lab - Vishal Korlipara 902504215 Genetics...

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Vishal Korlipara 902504215 Genetics Section D Applications of Drosophila melanogaster Introduction Drosphila melanogaster is a species of fruit flies that has been used as a criticial tool in the investigation of basic genetics. Drosophila are easily distingusihable and visible which allows for simple classification of phenotypes and sex. Drosophila males have a dark and round abdomen as well as sex combs. Female Drosophila have a colorless oval shaped abdomen with no sex combs. The small size of Drosophila make them ideal for classroom and laboratory settings becuase they are easy to cultivate without requiring a lot of room. Also Drosophila have a quick generation time of two days at 21 degrees celsius as well as a two week life cylcle which allows for a short-term project. Not only is the generation time very quick but also the amount of offspring is plentiful allowing for more detailed results. The last reason that Drosophila melanogaster are useful for laboratory experiments is that they have mutliple chromosomes and genes which makes them to a great representation of almost all living species in genetic studies. This experiment is being performed to examine if there is Mendelian independent assortment between the two traits: wing type (vg or vg + ) and body color (e or e + ). Also using statistical tests on the results can determine if the traits are located on the same chromosome or on different chromosomes and whether epitasis occured. This experiment will allow us to ascertain whether wing type and body color assort independently in Drosophila melanogaster . Hypothesis The null hypothesis is that there will be independent assortment with a 9:3:3:1 ratio. The nine would represent the wild type phenotype with normal color and normal wings. Each three would represent a parental genotype of either ebony with normal wings or normal color with vestigial wings. Lastly the 1 would represent the portion of flies that are ebony and vestigial. The alternate hypothesis would be that the genes are linked and epitasis has occured which resulted in an offspring ratio other than 9:3:3:1. Methods The experimenter recieved four Drosophila fruit flies from the F1 generation. After observation under the microscope the experimenter deduced that there were 3 normal winged (vg + vg), normal body colored (e + e) females and 1 normal winged (vg + vg), normal colored (e + e) male. They were placed into a vial with one scoop (about tablespoon) of media, one scoop (about a tablespoon) of water, and one pinch of yeast. The media was for yeast growth which the Drosophila larvae need for nutrition. The F1
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Drosophilia Lab - Vishal Korlipara 902504215 Genetics...

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