BIO POWER POINT

BIO POWER POINT - for staining of lipids. It is...

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Test for Solubility: Solubility in another Lipid or Fat
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The Negative and Positive Negative control. The test tube with the 2ml of water. Positive control The test tube with the 2ml of lipid
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Methods To test the solubility of Sudan IV or fat dye. A lipid will either dissolve or mix with a substance. Non-lipids form a layer on top of the tested substance (liquid).
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Lipids Are……… Hydrophobic (“water-hating”) Ester of fatty acids Utilized by living organisms
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What is Sudan IV? Sudan IV is a lysochrom (fat-soluble) used
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Unformatted text preview: for staining of lipids. It is reddish-brown. Maximum absorption is at 520(357) nm. Materials Sudan IV( Tested Substance) Test tube Salad oil Water Lipid Fat Results Test tube 1 has 2ml of water. (insoluble) Test tube 2 has 2ml of lipid. (soluble) Test tube 3 has 2ml of salad oil. (insoluble) Conclusion Test tube 2 shows the best solubility of the Sudan IV. This is because a lipid is consisted of three essential fatty chains....
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BIO POWER POINT - for staining of lipids. It is...

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