110225 - Molecularity of reaction (Chapter 9 324-332)...

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1 CHM3400 - Lecture 21 Feb 25 Renaturation of DNA Unimolecular (bi-, termolecular) reactions Reversible reactions Consecutive reactions Chain reactions Molecularity of reaction (Chapter 9 324-332)
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2 Renaturing of DNA Single-stranded DNA recombines to form double-stranded DNA: A + B AB [A] = [B], the rate becomes: rate = k[A][B] = k[A] 2 0 ] [ 1 ] [ 1 A kt A + = kt A A A 0 0 ] [ 1 ] [ ] [ + = Second-order reaction: f kt A A A = + = 0 0 ] [ 1 1 ] [ ] [ Dissociated fraction [A] = single-strand DNA [A] 0 = initial concentration of complementary strands
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3 Repeating units The concentration [A] 0 in the single-strand mixture depends on the complexity/ heterogeneity of the DNA sequence. For the extreme case: poly A – poly T, the repeating unit is 1 . Define C 0 = total concentration of single-strand DNA (A + B) before renaturation (of all sequences) N C A 2 ] [ 0 0 = N kt C f 2 / 1 1 0 + = Dissociated fraction: Repeating unit Only A
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4 Half-life and plots 1 2 2 / 1 0 = N k t C At the half-life of the reaction, f = ½ : ' 2 2 / 1 0 k N k N t C = = where k’ = k/2 N kt C f 2 / 1 1 0 + = Plot f vs. C 0 t determine f = ½
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110225 - Molecularity of reaction (Chapter 9 324-332)...

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