110408 - 1 CHM3400 - Lecture 35 Apr 8 Luminescence, lasers,...

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Unformatted text preview: 1 CHM3400 - Lecture 35 Apr 8 Luminescence, lasers, optical activity (Chapter 14 554-568) Fluorescence vs. phosphorescence Laser Refraction Rotation of polarized light Circular dichroism 2 Fluorescence Emission of radiation to return to the ground state (S ) from an electronically excited state (e.g. S 1 ). fluorescence > absorption Why? Mean lifetime of a fluorescent state (10-9 s) 3 Phosphorescence Comparison of phosphorescence vs. fluorescence: much slower process (10-3 s) spin-forbidden transition (triplet singlet) quantum yield? phos .. fluores Fluorescence yield F Photons absorbed Photons emitted = 4 Fluorescence imaging Endothelial cells: nuclei (blue), microtubules (green), actin (red) Selectively label different sites in cell with fluorescent labels to study dynamics of systems 5 LASER L ight a mplification by s timulated e mission of r adiation (short, intense burst of photons) Rate of stimulated absorption: ) 1...
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110408 - 1 CHM3400 - Lecture 35 Apr 8 Luminescence, lasers,...

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