Isolate Protein and determine the Concentration 2012.docx -...

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Isolate Protein and determine the Concentration BIOL 1205 Cells and Molecules Abstract
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In this lab we isolated protein from a sample of beef liver, and then we determined the protein concentration from a spectrophotometer. From the results we collected it was discovered that there is a distinct linear relationship between the amount of beef liver and its absorbance amount. The biuret test unquestionably pointed out the peptide bonds we were looking for in this experiment. As result we ended up working with 400mg of protein. Introduction Proteins are complex organic compounds. The constituents that make up proteins are carbon, hydrogen, nitrogen, oxygen and sulfur. Proteins are the only source of nitrogen in our body. Your body needs protein for growth, repair of tissues, immune response, hormone and enzyme production, muscle maintenance and energy. Its primary purpose is the growth and repair of body tissues. If the body's fat or carbohydrate stores are dwindling, it uses protein as an energy source. The body stores extra protein as fat or uses it immediately as an energy which is why its key. Proteins are made of long chains of amino acids that are connected by peptide bonds. This bond is formed from the condensation reaction and is considered stable. There are four levels of protein structure; the primary which is the linear arrangement of the amino acids, the secondary which has
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  • Fall '19
  • peptide bond

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