08.PMCB.lecture.8 - 9/10/2008 1 lant Molecular and Cellular...

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Unformatted text preview: 9/10/2008 1 lant Molecular and Cellular Biology ecture 8: Mechanisms of Cell Cycle Control and DNA Synthesis Gary Peter 9/10/2008 PMCB Lecture 9: G. Peter 2 Learning Objectives z Explain why a cell cycle was selected for during evolution z Explain the conceptual basis for cell cycle regulation z Explain the structure and function of the proteins that mediate cell cycle progression 9/10/2008 PMCB Lecture 9: G. Peter 3 Why Cell Cycle Control z Why was a cell cycle selected for? z Way to control cell size z Coordinate/control growth and development z Insure environment is favorable for replication and division and that each of the stages is completed prior to moving on to the next stage z Series of checkpoints insure completion of critical steps before progressing forward in cell cycle Genetic Strategy for Dissecting the Cell Cycle z Forward genetic approaches in Sacchromyces cerevisiae and Schizosacchromyces pombe were critical to identifying genes control cell cycle progression z Broad approach screening for cell division cycle mutants z Obtain collection of temperature sensitive lethals (nitrosoguanine) z Screen collection with time lapse photomicroscopy 9/10/2008 PMCB Lecture 9: G. Peter 4 9/10/2008 PMCB Lecture 9: G. Peter 5 enetic Dissection of the Cell Cycle: emperature Sensitive Mutants z Conditional mutants (temperature sensitive) isolated where cells arrest at specific cell cycle stages z Propagated at permissive temperature, z Restrictive temperature cycle progresses till the step at which mutant protein is needed for progression Genetic Analysis of Cell Division Cycle in S. cerevisiae Definition of a cell division cycle (cdc) mutant z Mutants that arrest cell division at a unique point no matter what stage of the cycle they were at when transferred from permissive to nonpermissive temperature z The fact that all cdc mutants arrested with the same mother-...
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This note was uploaded on 05/31/2011 for the course PCB 5530 taught by Professor Peter,g during the Spring '08 term at University of Florida.

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08.PMCB.lecture.8 - 9/10/2008 1 lant Molecular and Cellular...

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