08.PMCB.lecture9 - 9/10/2008 1 lant Molecular and Cellular...

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Unformatted text preview: 9/10/2008 1 lant Molecular and Cellular Biology ecture 9: Mechanisms of Cell Cycle Control Gary Peter 9/10/2008 PMCB Lecture 9: G. Peter 2 Learning Objectives z Explain why a cell cycle was selected for during evolution z Explain the conceptual basis for cell cycle regulation z Explain the structure and function of the proteins that mediate cell cycle progression z Explain the molecular mechanisms of the complex feedback loops z Explain the status of knowledge of G1 control and the plant cell cycle enopus Cell Free Extracts for Study- ng DNA Replication and Mitosis 9/10/2008 PMCB Lecture 9: G. Peter 3 http://images.google.com/imgres?imgurl=http://pasteur.crg.es/portal/pls/portal/docs/1/6240.JPG&imgrefurl=http://pasteur.crg.es/portal/pa ge/portal/0BA528148B1D8A26E04012AC0E017A42&h=327&w=472&sz=28&hl=en&start=12&um=1&tbnid=Du53hF007LTJ6M:&tbnh=89&tbn w=129&prev=/images%3Fq%3Dxenopus%2Bcell%2Bcycle%26svnum%3D10%26um%3D1%26hl%3Den%26client%3Dfirefox- a%26rls%3Dorg.mozilla:en-US:official%26sa%3DN urification of Maturation romoting Factor from Xenopus z A unit of MPF was defined as the minimum amount need to induce 20% of the pronuclei to enter M-phase a after 2h reaction 9/10/2008 PMCB Lecture 9: G. Peter 4 Lohka et al. 1988 PNAS 85: 3009-3013 Most purified preparations of MPF z Contains histone H1 kinase and autophosphorylation activities z Two main polypeptides co-elute with activity z Cyclin dependent kinase and cyclin 9/10/2008 PMCB Lecture 9: G. Peter 5 Lohka et al. 1988 PNAS 85: 3009-3013 urification of Maturation romoting Factor from Xenopus Biochemical Approach to Dissection z Immunodepletion and add back experiments z Cyclin depletion z Engineered proteins used to show ubiquitin degradation of cyclin B in mitosis 9/10/2008 PMCB Lecture 9: G. Peter 6 Effect of Cyclins on Chromosome...
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08.PMCB.lecture9 - 9/10/2008 1 lant Molecular and Cellular...

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