080818.PMCB.lecture.2

080818.PMCB.lecture.2 - lant Molecular and Cellular Biology...

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Unformatted text preview: lant Molecular and Cellular Biology Lecture 2: Fidelity of DNA Replication Gary Peter Learning Objectives 1. List and explain the mechanisms that insure high-fidelity DNA replication 2. Explain why the fidelity of DNA replication is so important for survival and for recombinant DNA work Importance of High-Fidelity DNA Replication z If 1 mutation occurs per 10 9 bp z Then what's the mutation rate of a 1000 bp gene in a population of 10 6 bacteria? z Whats the frequency of the mutant in the population? z Generation 1 z 10 6 copies of 1000 bp gene or 10 9 bp to be copied z 1 new mutant/ 2 x10 6 cells= 5x10-7 z Frequency in pop.=1/(2x10 6 )=5x10-7 z Generation 2 z 2x10 6 copies of 1000 bp gene or 2x10 9 bp to be copied z 2 new mutants/ 4x10 6 cells= 5x10-7 z Frequency in pop.=4/(4x10 6 )=1x10-6 z Generation 3 z 4x10 6 copies of 1000 bp gene or 4x10 9 bp to be copied z 4 new mutants/ 8x10 6 cells= 5x10-7 z Frequency in pop.=12/(8x10 6 )=1.5x10-6 z Mutation rate is constant at 5x10-7 z Frequency of mutants in the population increases Consequences of High Mutation Rates z Mutator strain propagated for various cycles 40 cycles = 1000 generations Funchain et al., 2000 Genetics 154: 959-970 What Processes and Pathways are Important for DNA Replication?...
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080818.PMCB.lecture.2 - lant Molecular and Cellular Biology...

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