080818.PMCB.lecture.3

080818.PMCB.lecture.3 - lant Molecular and Cellular Biology...

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Unformatted text preview: lant Molecular and Cellular Biology Lecture 3: DNA Polymerase Structure and Function Gary Peter Learning Objectives 1. List and explain the mechanisms that insure high-fidelity DNA replication 2. Describe and explain the structures and functions of the enzymes responsible for DNA replication DNA Polymerases: Function & Activities z FUNCTION z DNA replication & repair z ACTIVITIES z Polymerize dNTPs along a DNA or RNA template 5 to 3of the growing strand (Processively) z Exonuclease 5 > 3 z Exonuclease 3 > 5 2 nd Strand cDNA Synthesis DNA Polymerases Fall into Gene Families z Gene family identification relies on DNA sequence information and functional characterization of activities in vivo and in vitro z Different family members have diverse functions during replication, recombination & repair Phenotypes of E. coli with mutations in DNA Polymerase I z Mutations identified in the DNA polymerase I gene show that it is not required for DNA replication rather it is involved more in DNA repair z DNA polymerase III is the major enzyme involved in DNA replication Kornberg, DNA Replication, 1980 E. Coli DNA Polymerase Activities and Features Kornberg, DNA Replication, 1980 DNA Polyermase I Structure of DNA Polymerases 8/18/2008 PMCB Lecture 5: G. Peter 8 everse Transcriptase: Domain tructure Conserved Structure of DNA Polymerases Molecular Cell, Vol 8, 417-426, August 2001 DNA Polymerase Mechanisms are Conserved Sequence of Steps During Polymerization Binding DNA template-primer Binding of incoming dNTP Formation of phosphodiester bond Release of pyrophosphate Translocation of complex to the next 3...
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This note was uploaded on 05/31/2011 for the course PCB 5530 taught by Professor Peter,g during the Spring '08 term at University of Florida.

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080818.PMCB.lecture.3 - lant Molecular and Cellular Biology...

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