Learning Objectives1.List and explain the mechanisms by which E. coli DNA is replicated2.Describe and explain the structure and functions of the enzymes and their subunits that replicate DNA in E. coli
ProcessivityzThe number of nucleotides added during each binding and release from the primed templatezThe ability of the DNA polymerase to remain associated with the DNA templatezTypical processivity of enzymes in vitrozKlenow 50-60 ntzT7 300 ntzTaq 22 ntzPfu 6.4 ntWang et al., Nucleic Acids Res. 2004; 32(3): 1197–1207
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