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080818.PMCB.lecture.4 - Plant Molecular and Cellular...

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Plant Molecular and Cellular Biology Lecture 4: E. coli DNA Replicase Structure & Function Gary Peter
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Learning Objectives 1. List and explain the mechanisms by which E. coli DNA is replicated 2. Describe and explain the structure and functions of the enzymes and their subunits that replicate DNA in E. coli
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Processivity z The number of nucleotides added during each binding and release from the primed template z The ability of the DNA polymerase to remain associated with the DNA template z Typical processivity of enzymes in vitro z Klenow 50-60 nt z T7 300 nt z Taq 22 nt z Pfu 6.4 nt Wang et al., Nucleic Acids Res. 2004; 32(3): 1197–1207
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