Midterm 2010b - CHEM114C Midterm II Name _ May 18, 2010...

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CHEM114C Name ______________________________ Midterm II -1- May 18, 2010 ID#________________________________ Please write your name on each page. Your exam should have 5 pages including this cover page, with a total of 4 questions. Be certain that all work you want graded is written in permanent ink. You can use the back of the page to continue your answer. Do not use a calculator. This exam is 60 min long – work swiftly, most answers are short. GOOD LUCK! Your score 1. (30)_________ 2. (30)_________ 3. (30)_________ 4. (30)_________ Total (120)______________ This midterm counts 25% towards your final grade in this course.
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CHEM114C Name ______________________________ Midterm II -2- 1. Transcription is carried out by RNA polymerases. (30 total) 1.a) What is the subunit structure of the elongating E.coli RNA polymerase and which ion is located in the active site? (4) 1.b) Write down the polymerization reaction that is catalyzed by this enzyme. (4) 1.c) Diagram (on backpage) the transcription bubble indicating the directionality of sense and anti-sense DNA strands and the nascent RNA. (4) 1.d) Name the two footprinting techniques that reveal how the RNA polymerase interacts
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Midterm 2010b - CHEM114C Midterm II Name _ May 18, 2010...

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