Fungi in Culture: Not Mushroom
Before we even began our experiment, we observed all three stock cultures of the fungi which
were previously grown for approximately a week.
We looked at and recorded the date and time the culture
was grown, the texture, color, and the size of the colony we observed about the
, and the
After we recorded everything we thought was crucial
information about the three fungi, as a group we came up with a hypothesis in regards to two of them when
they are grown together.
Based on what we observed, we predicted that when
are grown together, the
will grow more abundantly in the petri
dish; however there will still be a distinct separation between the two fungi in all settings tested.
The first thing we did was use the wax pencils to label our petri dishes on the bottom with the
initials of every group member, the time, date, and which setting we were going to put the fungi in.
group we decided to use four different settings, one of which would be our controlled setting.
We chose to
put the fungi on a piece of wheat bread, have one in the light, one in the dark, and then one in normal room
temperature and light conditions, which was our control.
In order to get our piece of bread into the petri
dish we first put it in a piece of tin foil and smashed it up.
Next, we used the petri dish as a cookie cutter in
order to fit it inside.
By using the glass hockey stick, we were able to push the piece of bread down into the
petri dish without touching and/or contaminating it.
Once the petri dishes were ready, we could move on to obtaining the two fungi we decided to
use for the experiment, which again, were the
have got two, five milliliter test tubes, label each one with one of the fungi names using the wax pencil.
Fill the test tubes with three milliliters of dissociated medium, which is 2% glucose solution in a double