2005_campbl20

2005_campbl20 - Important Point Cloning Step in Overview...

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1 Chapter 20: DNA Technology and Genomics Important Point: DNA Technology ± DNA technology is the chemical manipulation of the genotypes and resulting phenotypes of organisms such that living organisms are modified ± Alternatively, no-longer-living organisms or their no- longer-living parts may be analyzed chemically at the level of genotype ± DNA technology has revolutionized how scientists study the genetics, biochemistry, even the ecology and evolutionary biology of organisms ± Genetic engineering is the artificial manipulation of the genetic material of organisms, including the creation of novel genetic material (i.e., novel nucleotide sequences) ± Biotechnology is the development of novel biological products, indeed whole industries are now devoted to the production and analysis of biological materials Cloning Step in Overview Have Transformant, then What? Clone Identification • Probing for correct DNA sequence • Probing for correct protein product • Antibiotic resistance β -galactocidase expression •E t c .
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2005_campbl20 - Important Point Cloning Step in Overview...

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