black06 - Microbial Growth Because individual cells grow...

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Unformatted text preview: Microbial Growth Because individual cells grow larger only to divide into new individuals, microbial growth is defined not in terms of cell size but as the increase in the number of cells, which occurs by cell division." Binary Fission Note nascent septum forming Planes of Division (1/3) Diplococcus Streptococcus Planes of Division (2/3) Tetrad Sarcinae Planes of Division (3/3) Staphylococcus Exponential Growth Note Logarithmic Scale Exponential Growth Standard Bacterial Growth Curve (1/2) Log Phase Continuous Culture Serial Transfer Generation Time Standard Bacterial Growth Curve (2/2) Decline (Death) Phase Link to Next Presentation Solid Medium Bacterial Colonies Standard Bacterial Count Colony-Forming Units Plaque-Forming Units Spread Plate Pour Plate Soft-Agar Overlay Soft Agar Overlay Spread & Pour Plates Dilutions and Limitations Serial Dilution Direct Microscopic Count Most Probable Number Method Most Probable Number Method Most Probable Number Calculations (based on 5 tubes/dilution) dilutions (10 = 1 ml culure) 10 10-1 10-2 10-3 10-4 MPN/ml 5 5 1 33 4 5 1 33 5 4 4 1 40 5 4 4 1 40 5 5 5 5 2 5400 1 0.20 4 4 1 1 4.7 Determination of Turbidity pH Optima Neutrophiles Acid-Based Microbial Succession...
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black06 - Microbial Growth Because individual cells grow...

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