black07 - leu+ ad+ Control ad+/leu+ Selecting for...

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DNA
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DNA
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Chromosomes, Eukarotic
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Chromosomes, Bacterial Note: Closed, Circular, Singular, and Haploid
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Gene E. coli = 5 million bp Phage T4 = 170,000 bp
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Gene
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Alleles Alleles simply are different Nucleotide Sequences found at the same Location on Different Chromosomes Usually the differences are quite subtle, e.g., one or two nucleotides different Big Difference between many Eucaryotes and most Bacteria: Bacteria, as Haploids, Express all of their Alleles
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Mutations New Alleles arise via Mutation The new Allelles may or may not be Functionally Different
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Mutations GAAGCGTT GAA A CGTT CTTCGCAA CTTCGCAA GAAGCGTT GAA A CGTT CTTCGCAA CTT T GCAA
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Prototrophic: microbes that live on a minimal growth medium + inorganic salts + energy source. Auxotrophic: mutant microbes must be supplied with certain additional nutrients to grow.
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Neurospora crassa Wt are ad + leu + Auxotrophs are: ad leu
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Unformatted text preview: leu+ ad+ Control ad+/leu+ Selecting for Auxotrophs • Prototrophic: Only dividing cells in minimal media Killed by penicillin Addition Prototrophs are killed • Auxotrophs Unaffected by penicillin because then are not dividing Wash cells, remove penicillin and grow auxotrophs! • Plate the auxotrophs on plates with various supplements to test for phenotypes Filtration Selection for Auxotrophs Selection for Prototroph Revertants Transcription Certain viruses have genes consisting of single-stranded RNA These viruses can be either plus-stranded or minus-stranded Plus-stranded RNA can serve as mRNA Minus-stranded RNA serve as templates for the synthesis of mRNA Transcription Link to Next Presentation Acknowledgements www.uta.edu/biology/mcallister/ 5312/download/14_Mutation.ppt homepage.smc.edu/bober_mary/Bio%2022/ gene%20mut%20web1.ppt...
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This note was uploaded on 06/03/2011 for the course MCB 205 taught by Professor Abedon during the Spring '11 term at Ohio State.

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black07 - leu+ ad+ Control ad+/leu+ Selecting for...

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