black08 - Sex! Bacteria Sex Transformation Transformation:...

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Sex!
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Bacteria Sex
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Transformation Transformation: DNA picked up directly from the medium and recombined into the genome heteroduplex Competent cell: capable of picking up DNA
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Transduction Viral products produced, host genome fragmented ~1 phage/10,000 will pick up chromosomal DNA. ..
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Plasmids
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Conjugation
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Resistance Plasmids
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Genetic Engineering
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Restriction Endonucleases A Restriction Endonucleases will cut both strands of a DNA duplex at a specific place These “places” need not be directly opposite: Note that this enzyme used is Eco RI, the first restriction endonuclease characterized 5’…GAATTC…3’ 3’…CTTAAG…5’ 5’…GAATTC…3’ 3’…CTTAAG…5’ 5’…G -OH P- AATTC…3’ 3’…CTTAA -P HO- G…5’
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Sticky Ends
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Ligation
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More RE Enzymes Enzyme Sequence Product Eco RI G^AATTC 5’ sticky ends Bam HI G^GATCC 5’ sticky ends Bg1 II A^GATCT 5’ sticky ends Pvu I CGATC^G 3’ sticky ends Pvu II CAG^CTG
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black08 - Sex! Bacteria Sex Transformation Transformation:...

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