nester02 - In lab well consider small (and some not so...

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Chapter 2: The Molecules of Life
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Important Point:
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Weak Bonds Holding Substances Together Weak Chemical Bonds form the Basis for the Existence of Life!
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Salt Dissolving in Water
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pH: acid < 7, base > 7
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pH: acid < 7, base > 7 To maintain the pH near neutrality, buffers typically are present in microbial growth media.
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Proteins/Protein Structure
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Proteins/Protein Structure Proteins only work properly if their “primary structure” = order of amino acids is correct. Proteins only work properly if their tertiary (or quaternary) structures are intact.
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Protein Denaturation: Destroys 2’, 3’, & 4’ structures High salt concentrations, detergents, reducing agents, etc. can also denature proteins.
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Lipid Bilayer Membranes are held together by weak intermolecular interactions between phosopholipids and other molecules including with proteins.
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Some Relevant Small-Molecules
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Unformatted text preview: In lab well consider small (and some not so small) molecules that affect bacterial growth. These include (but are not limited to): Glucose, Lactose, Sucrose (sugars/carbs) Mannitol (alcohol sugar) & Starch (both carbs) Citrate & Malonate (Kreb cycle intermediates) Nitrate (alternative final electron acceptor) Casein (milk protein) & Gelatin (also a protein) Amino acids (the units that make up proteins) Urea (nitrogenous waste removed by kidneys) Other substrates include more complex substances such as peptone & tryptose (both of which consist of partially digested proteins) . We will also consider a large number of salts, indicators (including pH indicators), pH buffers, metabolic products, & inhibitors of bacterial growth. Link to Next Presentation...
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This note was uploaded on 06/03/2011 for the course MCB 205 taught by Professor Abedon during the Spring '11 term at Ohio State.

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nester02 - In lab well consider small (and some not so...

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