nester04 - Chapter 4: Dynamics of Prokaryotic Growth...

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Chapter 4: Dynamics of Prokaryotic Growth
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Important Point:
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Each Species is Unique Bacteria are incredibly diverse, but. .. Each bacterial species can grow in only a limited set of environments. Each bacterial species can grow only if presented with the right nutrients/conditions. In addition, bacteria produce characteristic by- products (e.g., waste products). We can take advantage of these growth characteristics to identify bacteria phenotypically. To do these identifications we first have to get organisms in Pure Culture. Unfortunately, only about 1% of microorganisms currently can be grown in pure culture.
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Pure-Culture Basics Sterile = completely free of microbes. Aseptic Technique = procedures that minimize unintentional introduction of microorganisms to media (cultures) or from cultures to surrounding environment. Solid media is usually employed to obtain pure cultures. Agar is usually employed to make solid media. Agar melts at 95°C and solidifies below 45°C. Colony = pile of cells descended from single cell (or clump of cells). Petri Dish = container to which agar is added to obtain pure culture. Agar Plate (plate) = agar-containing petri dish.
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(mostly) Isolated Colonies
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Streak-Plate Method
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Storing Pure Cultures Stored pure cultures are often called “Stock Cultures” Stock cultures often are stored as/using: Frozen in glycerol solution Lyophilized = freeze drying On agar slants As stabs
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nester04 - Chapter 4: Dynamics of Prokaryotic Growth...

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