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Unformatted text preview: (Fig. 9.8) Restriction Fragment Length Polymorphism (RFLP) Analysis What is an RFLP? (fig. 9.9) Genomic DNA is isolated, cut using specific restriction enzymes, run out on a gel, and then Southern Blotted with specific DNA probes. Phages are viruses of bacteria. RFLP generation. Gels separate DNA by size. This is a postprobe detection blot. Phage Typing This is a “Soft-Agar Overlay.” Clonally related strains should have same RFLP type (i.e., same band pattern). The clear spots are due to bacteria killing (lysis). These are two strains that have been differentiated based on RFLP analysis. Why care about clonal relatedness? Is a contamination problem due to fault in your aseptic technique? Or is there just a particularly invasive bug making the rounds? “Phage” = “Bacteriophage” 4 Antibiogram The streaks make up a bacterial “lawn”. Numerical Taxonomy The disks are impregnated with specific antibiotics. G-C Content (DNA Base Ratio) Similarity Coefficients Rearranged into two distinct species... G-C Content (DNA Base Ratio) Numerical Taxonomy These two strains have different antibiotic-resistance patterns. Numerical Taxonomy is a method of classification that pools phenotypic characte...
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This note was uploaded on 06/03/2011 for the course MCB 205 taught by Professor Abedon during the Spring '11 term at Ohio State.

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