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Unformatted text preview: ar, etc.
Growth on Selective/Differential media: MacConkey
agar, EMB agar, etc.
Biochemical tests: “Most biochemical tests rely on a
pH indicator or chemical reaction that results in a
color change [or gas production] when a [specific]
compound is degraded.”
Keep in mind that to do well in lab it is important to gain
some reasonable understanding of how the various
biochemical tests work.
Note that “because each test often requires an
incubation period… it [is] too time-consuming to
proceed one [phenotypic characterization] step at a
time.” 2 Durham Tube: Gas Production Gas Generation: Catalase Test Commercial Biochemical Test Kits Color Change: Urease Test
Dichotomous Key Don’t worry about the
specific details of this
key but do keep in
mind how it is
organized. Typing of Strains
Biovar, Biotype (syn) Serovar, Serotype (syn) Typing via RFLP analysis Ribotype (RFLP of rRNA) Also Phage Type 3 1 234 5 Can you tell me
which lanes contain
bacteria that are
clonally related? What is an RFLP? (fig. 9.9)
Different strains have
recognition sites in
different places due
differences. Note resulting
length. Ribotyping Southern Blotting...
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This note was uploaded on 06/03/2011 for the course MCB 205 taught by Professor Abedon during the Spring '11 term at Ohio State.
- Spring '11