HW 1 - max and K m What information does each variable...

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
Problem Set #1 How do Enzymes Work? 1. What was the first enzyme to have its three-dimensional structure determined by x-ray crystallography? How did this achievement support transition-state theory? 2. Although the serine, histidine, and aspartate residues present within the serine protease catalytic triad play a major role in catalysis, mutational analysis studies have shown that catalysis still occurs when these residues are replaced by alanines. Explain this observation. 3. Draw a Michaelis-Menten plot and label the following variables: V
Background image of page 1
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: max and K m . What information does each variable provide about enzyme catalysis? 4. As mentioned in lecture, one unit of acetylcholine esterase catalyzes the hydrolysis of acetylcholine 25,000 times per second. Comparing this observation to carbonic anyhydrase (600,000 times per second) it seems unlikely that their catalytic efficiencies are similar, however, they are in the same ballpark. Explain this observation in terms of Michaelis-Menten variables....
View Full Document

This note was uploaded on 06/02/2011 for the course CHEM 157 taught by Professor burkart during the Spring '07 term at UCSD.

Ask a homework question - tutors are online