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Lab - Ayyam Ibrahim Chemistry 1M Determination of Allura...

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Ayyam Ibrahim May 13, 2011 Chemistry 1M Determination of Allura Red Concentration in Mouthwash Abstract: Allura red, a dye in some commercial products (mouthwash), was measured using a Spec 20 spectrometer; in order to determine the wavelength of light absorbed most strongly by allura red. With the calculated absorbance values, a Beer’s Law plot of absorbance versus concentration was constructed. This lab was divided into three parts; in the first part the (λmax) was found to be 505 nm. In part 2, a number of volumetric dilutions were made in order to find transmittance and absorbance for various known concentrations of Allura red. Also, these calculations were used to make a Beer’s law plot; the slope of the plot is 24,840nm. Finally, in part 3, it was required to determine the concentration of Allura red in mouthwash, which was determined to be 1.45E-45M. Results:
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The (λmax) is 505nm Λ %T T Absorbance 400 53 .53 .2757 420 49.5 .495 .3054 440 42 .42 .3767 460 26.5 .265 .5767 480 14.5 .145 .8386 500 11 .11 .9586 520 13.5 .135 .8697 540 26 .26 .5850 560 56 .56 .2518 580 87 .87 .0605 600 96 .96 .0177 620 98 .98 .0088 640 94 .94 .0269 660 92 .92 .0362 680 88 .88 .0555 700 85 .85 .0757
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