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Answers+assign1 - BIS 104, Section 2, Winter quarter 2011...

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BIS 104, Section 2, Winter quarter 2011 Answers to Assignment 1 Note: Answers are shown in red beneath each question. 1) You are attempting to view a cell using a light microscope. The limit of resolution you can achieve using visible light of 550 nm wavelength is 0.6 µ m. If you use the same lenses of this microscope in fluorescence microscopy using an ultraviolet wavelength of 365 nm, will the resolution be improved, made worse, or stay the same? Why? The resolution will be improved. The limit of resolution (d) is determined by the following equation: Resolution d = .61 λ / η sin θ Since the wavelength of ultraviolet light is less than the wavelength of visible light, the numerator of this equation would be reduced, thus lowering the value of d. The smaller the value of d, the better the resolution. 2) Protein X is an integral membrane protein that makes a single pass through the membrane of a certain type of cells. You have antibodies against protein X and have labelled these antibodies with a green fluorescence dye called fluorescein. You treat a sample of cells with the fluorescein-labeled anti-X antibodies. If you conduct this
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This note was uploaded on 06/02/2011 for the course BIS 104 taught by Professor Scholey during the Winter '08 term at UC Davis.

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Answers+assign1 - BIS 104, Section 2, Winter quarter 2011...

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