Lecture 21+22

Lecture 21+22 - Lecture 21 Molecular Techniques DNA...

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Lecture 21 Molecular Techniques DNA cloning: General Strategy Study structure and functions of genes at molecular level requires a large quantity of individual genes in the pure form DNA cloning : Prepare large quantities of identical DNA molecules Taking one exact genome and reproducing the exact same one Recombinant DNA : Any DNA molecule composed of sequences derived from different sources New DNA that did not originally exist in nature Key process : Link to a vector DNA Vector : Molecule used to transfer foreign genetic material into another cell Molecule that can replicate without a host cell Summary:
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Vectors can propagate in bacteria or in eukaryotic cells such as yeast DNA fragment can be of any origin of the same species o No way for transcriptional machinery to distinguish between eukaryotic base pair or a more primitive form Plasmids : Long pieces of circular DNA that exist natural in bacteria
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This note was uploaded on 06/05/2011 for the course BIO 200 taught by Professor Thomasebureau during the Fall '07 term at McGill.

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Lecture 21+22 - Lecture 21 Molecular Techniques DNA...

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