Biotechnology Matt(1)

Biotechnology Matt(1) - Biotechnology Biotechnology The use...

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Biotechnology
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Biotechnology The use of microorganisms, or biological substances, such as enzymes, to perform specific industrial or manufacturing processes
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Science Fact or Fiction? http://www.flickclip.com/flic ks/jurassicpark3.html
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The process of producing modified DNA in a test tube and reintroducing that DNA into host organisms. Genetic Engineering
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Synthetic Biology Colonies of the transformed Mycoplasma mycoides bacterium. Image Credit: J. Craig Venter Institute.
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1. Gene Therapy (24.5) Dogs with the genetic disease mucopolysaccharidosis VII (MPS VII) - enzyme deficiency leads to clouding of corneas, cardiac disease, bone abnormalities - retroviral vector for neonatal gene therapy Gene therapy No gene therapy http://www.vet.upenn.edu/schoolresources/communications/publications/bellwether/55/gene_therapy.html
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2. Transgenic plants (24.2) + = Bacillus thuringiensis corn cry gene Bt-Corn European corn borer
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3. Transgenic Animals (24.3) GloFish® Assortment + = Zebra fish jellyfish gfp gene
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4. Bioremediation Oil-eating bacteria to clean up toxins in the environment Microorganisms are eating creosote and chlorinated phenols in Pensacola, FL http://www.clarkson.edu/news/view.php?id=1095
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5. Forensics http://www.king-george.va.us/content6.cfm?cont_uid=2 http://www.guardian.co.uk/science/2007/se p/16/sciencenews
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6. Pharmaceutical companies http://www.bio.org/speeches/pubs/er/
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7. Research • Understanding gene expression, developmental genetics, cancer, aging, genomes, etc. • Nearly all labs use some form of biotechnology
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Tools of the Trade
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PCR: DNA amplification in vitro • Once you know the sequence of part of a gene, you can amplify it P olyermase C hain R eaction Kary Mullis winner of the Nobel Prize in chemistry
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11-21
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11-21
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11-21 http://highered.mc graw- hill.com/sites/007 2437316/student_ view0/chapter16/a nimations.html# PCR video
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This note was uploaded on 06/08/2011 for the course PCB 3063 taught by Professor Marta during the Spring '08 term at University of Florida.

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Biotechnology Matt(1) - Biotechnology Biotechnology The use...

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