handout 2.15.11 - 1 Homologous recombination Standard...

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1 2/15/11 1. Homologous recombination – Standard mechanism of crossover, which is mediated through the sequence similarity between the DNAs. As such, limited to shared regions between the DNAs 2. Nonhomologous recombination – Less standard mechanism of crossover, which is not dependent on shared sequence similarities between the DNAs. As such, can occur at many more sites between the DNAs 3. Plaque – Visible hole in bacterial lawn on Petri dish, representing the clonal descendants of a single progenitor phage 4. Complementation test – If two mutants can reconstitute the normal wild type, then their mutations reside in different genes. If not (or in any rarely so), then their mutations occur within the same gene 5. Molecular complementation – Two mutants occurring at different sites within the same gene can crossover to reconstitute (but only very rarely) the normal wild type. Thus, crossing over can occur within a gene as the gene is not indivisible 6. Replicon – Segment of DNA under the control of the same replication origin; a unit of DNA replication --------------------------------------------------------------------------------------------------------------------------------------- RNA VIRUSES Class 3 – Double stranded RNA (dsRNA) viruses Class 1 – Single stranded RNA (ssRNA) viruses, where ssRNA functions directly as both genome and mRNA Class 2 – ssRNA viruses, where ssRNA functions directly as genome and template for mRNA
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This note was uploaded on 06/08/2011 for the course PCB 3063 taught by Professor Marta during the Spring '08 term at University of Florida.

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handout 2.15.11 - 1 Homologous recombination Standard...

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