2010%20Final

2010%20Final - CHEM114C Final Name _ June 10, 2010 ID#_...

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CHEM114C Name ______________________________ Final -1- June 10, 2010 ID#________________________________ Location Code___________________________ Please write your name on each page. Your exam should have 9 pages including this cover page, with a total of 8 questions. Be certain that all work you want graded is written in permanent ink. You can use the back of the page to continue your answer. Do not use a calculator. This exam is 120 min long – work swiftly, most answers are short. GOOD LUCK! Your score 1. (26)_________ 2. (24)_________ 3. (26)_________ 4. (26)_________ 5. (28)_________ 6. (26)_________ 7. (26)_________ 8. (26)_________ Total (208)______________ This FINAL counts 50% towards your final grade in this course. If you opted out of one Midterm, it counts for 75%. If you opted out of both Midterms, it counts for 100%.
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CHEM114C Name ______________________________ Final -2- 1. DNA Recombination (26) 1.1. A critical step in homologous recombination involves the Holliday junction. Diagram show how the Holliday junction can be resolved in two possible ways. (8) 1.2. During E.coli DNA synthesis the replication fork suddenly encounters an unrepaired nick in the DNA. The replication fork collapses, but a recombination repair mechanism saves the bacterium. Diagram (on backpage) the recombination repair mechanism and indicate the proteins involved in it. (8) 1.3. In humans, aside from repair, what other physiological roles does DNA recombination have? Name two, and state in one sentence what recombination achieves. (4) 1.4. In the evolution of the human genome, homologous recombination has also played important roles. Describe briefly one example that is beneficial, one that seems neutral, and one that is potentially harmful for the organism’s fitness. (6)
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CHEM114C Name ______________________________ Final -3- 2. Gene Structure (24) Draw the following 3 diagrams underneath each other and indicate any relationships between them: 2.1. A typical eukaryotic gene indicating the enhancer, core promoter with TATA box, exons,
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2010%20Final - CHEM114C Final Name _ June 10, 2010 ID#_...

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