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Unformatted text preview: six affected persons have the 3 and the 2 kb bands, but no unaffected person have them; therefore these fragments are likely to be linked to the disease allele (in cis arrangement). Because 3 plus 2 adds up to 5, a likely arrangement in the father is as follows, where D is the disease allele and an arrowhead represents the restriction-enzyme target site: The mother is as follows: b. The last son most likely represents a crossover between the disease locus and the RFLP (restriction fragment length polymorphism) locus, resulting in a chromosome with D and the 5 kb fragment. c. Because the 3 and 2 kb fragments are linked to the disease allele, the presence of these fragments can be used as a predictor for the disease. However, the diagnosis has to take into account the possibility of a crossover....
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