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Unformatted text preview: 3-T TCAGCATTGGAACGTG CCATCG-5 Forward Reverse A) 5-AACCTT-3 5-AAGTCG-3 B) 5-TTCAGC-3 5-CGGGGC-3 C) 5-AAGTCG-3 5-GCTACC-3 D) 5-GGTAGC-3 5-GCCCCG-3 E) 5-ATGCGT-3' 5-TTGGAA-3' C) The forward 5 to 3 primer would be complimentary to the left hand 3 end. The primer will appear as the non-template strand of DNA. When we do the same for the other 3 end, we get the reverse of the non-template strand of DNA. 4. Design primers that will amplify the following region of DNA (assume this is one strand from a double stranded region of DNA). The primers should be 15 bases in length in 5 3 orientation. 5- GGATCGATCAAGAACAATGACAGGATCGAGGAATTCAGCCTACGCAGCGAGTAGCTGGAGGGA-3 3- CT CATCGACCT CCCT-5 5- GGATCGATCAAGAAC-3 3- CC TAGCTCGTTCTTGTTACTGTCCTAGCTC CTTAAGTCGGATGCGTCGCTCATCGAGGTCCCT-5 In 5-3 orientation the primers are: F: GGATCGATCAAGAAC R: TCCCTCCAGCTACTC...
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