1Electrophoresis - DNA Analysis Techniques 1. 2. 3. 4. 5....

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DNA Analysis Techniques 1. Measuring DNA length 2. Copying DNA 3. Cutting and Pasting DNA 4. Probing DNA 5. DNA Cloning
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Measuring DNA Length An Introduction to Bioinformatics Algorithms www.bioalgorithms.info
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Gel Electrophoresis separating DNA segments according to size The DNA sequence can be read by a technique called gel electrophoresis which separates DNA molecules into groups depending on their lengths. Gel electrophoresis has high resolution; even fragments which differ by a single nucleotide can be separated. The sample molecules are placed in a gel under the influence of an electric field .
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Gel Electrophoresis(contd.) The DNA or RNA molecules (which are slightly negatively charged) can migrate towards the positive electric field. The speed of migration is inversely proportional to the length of the molecule; longer molecules move slow, shorter move faster. All molecules are initially placed at the top of the ‘well’ and after a few hours, the molecules move to different
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1Electrophoresis - DNA Analysis Techniques 1. 2. 3. 4. 5....

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