3PCR - An Introduction to Bioinformatics Algorithms...

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Copying DNA PCR (Polymerase Chain Reaction) An Introduction to Bioinformatics Algorithms www.bioalgorithms.info
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Why we need so many copies Biologists needed to find a way to read DNA codes. How do you read base pairs that are angstroms in size? It is not possible to directly look at it due to DNA’s small size. Need to use chemical techniques to detect what you are looking for. To read something so small, you need a lot of it, so that you can actually detect the chemistry. Need a way to make many copies of the base pairs, and a method for reading the pairs.
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Molecular Biology Laboratory Techniques :Polymerase Chain Reaction (PCR) Restriction enzymes and plasmid cloning techniques are used routinely in many laboratory experiments. The discovery of PCR has replaced these techniques for large scale sequencing of genomes. Without PCR automated and fast sequencing technology would not have been possible.
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PCR is a cell-free method of amplifying a short (<15kb)
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This note was uploaded on 06/12/2011 for the course CAP 5510 taught by Professor Staff during the Spring '08 term at University of Central Florida.

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3PCR - An Introduction to Bioinformatics Algorithms...

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