Cloning - An Introduction to Bioinformatics Algorithms...

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Cloning 1 An Introduction to Bioinformatics Algorithms
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Recombinat DNA Technology or Genetic Engineering In the 1950s and 60s, the Biologists used biochemical, biophysical and crystallographic procedures to discover the double helix structure of DNA. But, they were frustrated not being able to examine a small fragment of DNA such as a gene in pure and isolated form to perform critical experiments to discover the detail working of a gene. This situation led to the discovery of the the recombinant DNA technology or genetic engineering in the 1970s. At the core of this new technology was gene cloning . In order to study a specific fragment of DNA sequence, we need to select the fragment and amplify it so that the solution contains a purified near-homogeneous population. 2 An Introduction to Bioinformatics Algorithms
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3 An Introduction to Bioinformatics Algorithms The basic steps of the gene cloning experiment is as follows: 1 . A fragment of DNA containing the gene to be cloned is inserted into second( usuallyCircular) DNA molecule called a vector. 2.
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This note was uploaded on 06/12/2011 for the course CAP 5510 taught by Professor Staff during the Spring '08 term at University of Central Florida.

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Cloning - An Introduction to Bioinformatics Algorithms...

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