Determining gene function•Sequence comparison tells us if a gene is similar to another gene, e.g., in a new species–Dynamic programming–Approximate pattern matching•Genes with similar sequence likely to have similar function•Doesn’t always work.–“Homologous” genes may not be similar enough at the sequence level, to be detected this way•New method to determine gene function: directly measure gene activity (DNA arrays)
DNA Arrays--Technical Foundations•An array works by exploiting the ability of a given mRNA molecule to hybridize to the DNA template. •Using an array containing many DNA samples in an experiment, the expression levels of hundreds or thousands genes within a cell by measuring the amount of mRNA bound to each site on the array. •With the aid of a computer, the amount of mRNA bound to the spots on the microarray is precisely measured, generating a profile of gene expression in the cell. May, 11, 2004http://www.ncbi.nih.gov/About/primer/microarrays.html3An Introduction to Bioinformatics Algorithmswww.bioalgorithms.info
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