DNASequencinglink - DNA Sequencing link:

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DNA Sequencing link: http://en.wikipedia.org/wiki/DNA_sequencing DNA sequencing From Wikipedia, the free encyclopedia Jump to: navigation , search The term DNA sequencing encompasses biochemical methods for determining the order of the nucleotide bases, adenine , guanine , cytosine , and thymine , in a DNA oligonucleotide . The sequence of DNA constitutes the heritable genetic information in nuclei , plasmids , mitochondria , and chloroplasts that forms the basis for the developmental programs of all living organisms. Determining the DNA sequence is therefore useful in basic research studying fundamental biological processes, as well as in applied fields such as diagnostic or forensic research. The advent of DNA sequencing has significantly accelerated biological research and discovery. The rapid speed of sequencing attained with modern DNA sequencing technology has been instrumental in the large-scale sequencing of the human genome , in the Human Genome Project . Related projects, often by scientific collaboration across continents, have generated the complete DNA sequences of many animal, plant, and microbial genomes. DNA Sequence Trace Contents [ hide ] 1 Early methods 2 Maxam-Gilbert sequencing 3 Chain-termination methods o 3.1 Dye-terminator sequencing o 3.2 Challenges o 3.3 Automation and sample preparation 4 Large-scale sequencing strategies 5 New sequencing methods o 5.1 High-throughput sequencing o 5.2 Other sequencing technologies
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6 Major landmarks in DNA sequencing 7 See also 8 Citations 9 External links [ edit ] Early methods For thirty years, a large proportion of DNA sequencing has been carried out with the chain- termination method developed by Frederick Sanger and coworkers in 1975. [1][2] Prior to the development of rapid DNA sequencing methods in the early 1970s by Sanger in England and Walter Gilbert and Allan Maxam at Harvard , [3][4] a number of laborious methods were used. For instance, in 1973 [5] Gilbert and Maxam reported the sequence of 24 basepairs using a method known as wandering-spot analysis. RNA sequencing, which for technical reasons is easier to perform than DNA sequencing, was one of the earliest forms of nucleotide sequencing. The major landmark of RNA sequencing, dating from the pre-recombinant DNA era, is the sequence of the first complete gene and then the complete genome of Bacteriophage MS2 , identified and published by Walter Fiers and his coworkers at the University of Ghent ( Ghent , Belgium ), published between 1972 [6] and 1976. [7] [ edit ] Maxam-Gilbert sequencing In 1976-1977, Allan Maxam and Walter Gilbert developed a DNA sequencing method based on chemical modification of DNA and subsequent cleavage at specific bases [1] . Although Maxam and Gilbert published their chemical sequencing method two years after the ground-breaking paper of Sanger and Coulson on plus-minus sequencing, [8][9] Maxam-Gilbert sequencing rapidly became more popular, since purified DNA could be used directly, while the initial Sanger
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This note was uploaded on 06/12/2011 for the course CAP 5510 taught by Professor Staff during the Spring '08 term at University of Central Florida.

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DNASequencinglink - DNA Sequencing link:

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